Human embryonic stem cells are pluri – potent cell lines usually derived from human blastocysts. Their potential critically depends on longterm proliferative capacity, developmental potential after prolonged culture, and karyotypic stability. Cell viability is an important parameter for assessing cell sample quality. Here, we elaborate the stored human embryonic stem cell lines’ viability in a ready to use form for a period of 9 years (from 2007 to 2015).
We observed high cell viability in our cell lines for 9 years. Human embryonic stem cell lines in a ready-to-use form can be preserved for longterm purposes. Thus, they could be made available globally.